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Bacterial cells were harvested by carefully removing the supernatant by centrifugation (1,000 g , 10 min, 4 C) and the resulting pellet was resuspended with 50 l of B-PER reagent (supplemented with 1 mM DTT and 1 mM PMSF)

T cells were cultured in human T cell medium (HTCM) consisting of X-Vivo 15 (Lonza, 04-418Q), 5% human AB serum (Fisher Scientific, NC9310328) and 10 mM neutralized N -acetyl-l-cysteine (Sigma-Aldrich, A9165) supplemented with 30 U per ml IL-2 (PeproTech, 200-02 or BRB preclinical biologics repository, National Institutes of Health (NIH)) at 37 C under 5% CO 2

Direction For Use -Drop the tablet in a glass of water

The accumulation of mtDNA mutations with aging and disease jeopardizes respiratory capacity, leading to bioenergetic failure, oxidative stress, and neuronal vulnerability